A Secret Weapon For attune flow cytometer
A Secret Weapon For attune flow cytometer
Blog Article
At that time, the early symptoms ended up dismissed as debris, nevertheless the retrospective evaluation demonstrated shared characteristics Using the problematic cells in culture. Tracing the infection helped the lab build extra laboratory strategies for screening and security of assay-critical cell traces.
As samples are obtained around the Attune CytPix Flow Cytometer, the high-pace brightfield camera captures and suppliers illustrations or photos of detected activities at a charge up to 6,000 images for each 2nd, depending upon the flow fee and picture dimensions. For bigger flexibility, Attune Cytometric Computer software helps you to regulate the graphic capture frequency as essential and select a particular gate for picture seize, if expected.
Mix acoustic focusing technological innovation with substantial-parameter analysis for adaptable spectral unmixing and standard workflows.
Learn new insights into your experimental information by visualizing populations using mixed fluorescence and morphology facts. Our software package interface wizards allow downsampling, concatenation (merged files) and dimensionality tools which might be built into the program for an intuitive, streamlined info Assessment practical experience.
"An image is not pretty truly worth 1000 dots, but it surely definitely helps to see photos. It is excellent to see photos that correlate with staining. Such as, AnnexinV-constructive cells that are morphologically distinct than nutritious cells, or CD14+ monocytes that are bigger and even more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, a seasoned user has gated singlets confidently. Just after analyzing the guide singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate has over four% aggregates.
Optimizing gating system. Even strong handbook singlet gating is error-prone and stays a subjective determination issue in Pretty much all flow cytometry assays. Imaging may be used to substantiate and alter gates to incorporate only solitary cells of curiosity.
Consistent picture high quality even at substantial throughput Acoustic focusing plus a higher-pace digital camera Mix to image these T cells regularly at low or significant flow fees. Easily regulate concentrate and digital camera options to fulfill experimental needs.
The Attune NxT cytometer will be the trustworthy workhorse instrument your lab can depend upon for reliable and trustworthy general performance. The Attune CytPix design incorporates a large-speed brightfield digicam and AI-driven image analysis program, with modern updates like equipment Finding out tools and an intuitive consumer interface, enabling Increased knowledge precision, accuracy, and more rapidly insights into sample biology.
Consumers can check out party photographs in true-time to verify sample good quality, optimize protocols, and history the morphology of every cell inhabitants recognized for Investigation. Imaging seize also Gains from acoustic focusing, which helps you to position the cells to make sure that a pointy, centered graphic is received.
Audio waves empower precise mobile alignment, leading to Fantastic info high-quality and ~10X more quickly sample flow fees.
We Formerly demonstrated the strength of imaging Auto-T/Ramos cell interactions. Let’s look at just the inhabitants of finest interest, the double optimistic occasions, to learn more. We can easily now use extended morphological parameters (below, circularity and skewness of depth) to even further look at the features of these populations and refine gating on these functions, escalating knowledge robustness.
Leverage the power of Superior engineering and innovation to speed up your discoveries. Believe in while in the Attune Flow Cytometer loved ones to provide the reliability, pace, and accuracy that set your get the job done aside.
No clean/no lyse leukocyte Assessment with imaging. Cells get more info have been acquired from 24-hour-aged blood by dilution in one mM EDTA ( Qualified UX/UI allows people to coach AI models for expanded programs. (A) The “Teach Product” element supplies annotation resources and real-time design instruction precision.
At the guts of each and every Attune Flow Cytometer lies our innovative acoustic focusing technological know-how, which makes use of audio waves to meticulously align cells throughout the flow cell. This innovative tactic surpasses regular hydrodynamic strategies, leading to more rapidly processing speeds, Excellent knowledge good quality, and enhanced sample throughput.
The Attune Xenith cytometer is really a superior-parameter spectral analyzer, delivering each spectral unmixing and conventional flow capabilities for thorough and versatile cell Examination.
The Attune CytPix Flow Cytometer makes it possible for end users to visualise samples throughout acquisition, validate the precision of gating approaches, and assist benefits with reputable documentation for publication reasons.
Right here, we present that by gating strategies employing image-primarily based quantitative parameters can distinguish interacting cells from coincident situations to much more properly review interacting cells.